Performance Peptide Lab
Lab Peptide Mixing Calculator
Quickly calculate the exact amount of diluent to add to your peptide vial and the precise volume to measure for your selected dose.
How It Works
Three Steps to Accurate Measurement
Enter Vial Details
Select the mg amount printed on your peptide vial and how much BAC water you’re adding.
Set The Dose
Enter your desired dose in mg or mcg – toggle between units. The calculator handles the rest instantly.
Read the Syringe
A visual syringe scale shows the exact tick mark to draw to – no manual maths required.
Free Tool
Mixing & Dosage
Supports reconstitution and injection dose calculations for all common vial sizes.
Background
Reconstitution:
Peptides are supplied as a lyophilised (freeze-dried) powder in sealed vials. Before use in laboratory research, the powder must be dissolved in a sterile solvent – most commonly bacteriostatic water (BAC water) – a process known as reconstitution.
The volume of BAC water added directly determines the concentration of the resulting solution (measured in mg/mL). A higher water volume produces a more dilute solution; less water produces a more concentrated one.
Once the concentration is known, the exact draw volume for any given dose can be calculated – which is precisely what this tool does.
Key Formula
Concentration
(Peptide mg x 1000) / BAC water mL
= mg per mL
Draw Volume
Desired dose (mg) / Concentration
= mL to draw
Syringe Units (IU)
Draw volume (mL) x 100
= Units / Ticks on a 1mL / 100IU syringe
FAQ
Frequently asked questions
What is bacteriostatic water and when is it used?
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Bacteriostatic water is sterile water containing 0.9% benzyl alcohol, which inhibits microbial growth. It is commonly used as a diluent in laboratory peptide reconstitution where multi-use handling may be required.
How much diluent should be added to a peptide vial?
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The appropriate volume depends on the target concentration and measurement requirements. For example, adding 1–2 mL to a 5 mg vial yields a concentration of 2.5–5 mg/mL. Use the calculator above to determine a dilution that aligns with your required measurement precision.
What do IU markings represent on a syringe?+
IU (International Units) markings on insulin syringes correspond to volume, not biological activity in this context. A standard 1 mL syringe contains 100 IU markings (1 IU = 0.01 mL), while a 0.5 mL syringe contains 50 IU markings. The calculator converts volume (mL) into corresponding syringe units for accuracy.
How much diluent should be added to a peptide vial? How much diluent should be added to a peptide vial?
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The appropriate volume depends on the target concentration and measurement requirements. For example, adding 1–2 mL to a 5 mg vial yields a concentration of 2.5–5 mg/mL. Use the calculator above to determine a dilution that aligns with your required measurement precision.
What is the difference between the two calculator tabs? +
Reconstitution – use this when you are mixing a new vial. It tells you exactly what mark to draw to based on the water volume you add.
Measurement Mode – Use this when the vial has already been reconstituted. Enter the vial concentration and required quantity to determine the corresponding draw volume, along with calculated usage totals and estimated vial utilisation over time
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